Akt Pure 2/PKBa/Akt 150ug

Protein Kinase B (PKB), also known as Rac (Related to the A and C kinases) Protein Kinase, shows 73% and 68% to Protein Kinase C

(PKC) and Protein Kinase A (PKA), respectively. It was also identified as the product of the v-akt oncogene from T-cell lymphomas.

Akt/PKB mRNA has been reported to be expressed in a wide variety of cell lines and has been detected in porcine brain, heart, liver, kidney,

muscle, and ovary. Rac phosphorylates a number of physiological substrates including MBP, glycogen synthetase, PKA RII subunit, and

histone H1. Akt/PKB is activated in response to growth factors through the activation of PI3-kinase and Ras. Activation of PI3-Kinase

generates phosphatidylinositol 3,4-bisphosphate which may induce the membrane translocation of Akt/PKB coincident with its

phosphorylation and activation. Cellular stress leading to the activation of the p38 MAP kinase also induces Akt/PKB activation, indicating a

multiplicity of signaling pathways that activate Akt/PKB. Upon activation, Akt/PKB associates with some members of the PKC family of

kinases, such as PKCδ and PKCζ. Downstream of Akt/PKB, GSK3 is thus far the only identified substrate for Akt/PKB phosphorylated in

vivo.

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.