SLP-76 Pure 8/SLP-76 50ug

SLP-76 (SH2 domain-containing Leukocyte Protein) is a tyrosine phosphoprotein that is involved in the TCR mediated intracellular signaling

pathway. It may be involved in the signaling pathways of peripheral blood leukocytes, thymic/splenic cells, and in human T, B, and monocytic

cell lines. SLP-76 consists of several motifs that signify its importance in protein-protein interactions involved in intracellular signaling

pathways, such as the SH2 domain in the C-terminus, the amino-terminus three 17-amino acid repeats with conserved tyrosine and acidic

residues (DYE(S/P)P), and a proline rich region. SLP-76 has been shown to associate with Grb2, PLC-γ, SLAP-130, and Vav-all of which are

part of the signaling cascade in T cells. ZAP-70 appears to phosphorylate SLP-76, allowing phosphorylated Vav to bind and, together the

SLP-76/Vav complex, cooperatively stimulates NF-AT and IL-2 gene activation. Overexpression of SLP-76 has been reported to increase the

TCR-mediated transcription of the IL-2 gene. Furthermore, SHP-1 associates with SLP-76 in normal splenic B cells which suggests a role for

SLP-76 in BCR signaling.

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.