Rab5 is a low molecular weight GTP-binding protein that plays a role in endocytic vesicle traffic. Like other Rab proteins, Rab5 has
C-terminal cysteine residues that are post-translationally modified by geranylgeranylation which is critical for its membrane targeting. Rab5 is
associated with early endosome and plasma membranes and evidence suggests that Rab5 regulates early endosome fusion. The GTP/GDP
cycle controls shuttling of Rab proteins between the cytosol and membranes. In vitro, Rab5 proteins are removed from membranes by a GDP
dissociation inhibitor protein (rabGDI) which leads to the formation of a cytosolic Rab5-rabGDI complex. Rab5 may insert into membranes by
a multistep process in a which a transient GDP-Rab5 intermediate is formed and converted into GTP-Rab5 that subsequently enters the
acceptor membrane and releases rabGDI into the cytosol.
原厂资料：
注意事项：
1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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