Nm23 Pure 56/Nm23 50ug

Nm23 is a potential metastasis suppressor protein whose expression is either reduced, altered (by mutation), or amplified in various types of

metastatic carcinomas. Two human gene homologues, nm23-H1 and nm23-H2, encode 17 kDa proteins that are 90% identical. Nm23 proteins

possess a nucleoside diphosphate kinase (NDPK) activity. This enzymatic activity catalyzes the synthesis of non-adenine-containing

nucleoside triphosphates from nucleoside diphosphates via a phosphorylated enzyme intermediate. In addition, Nm23 inhibits differentiation,

interacts with GTP-binding (GAP) proteins, autophosphorylates serine residues, and binds to DNA. The biochemical mechanisms by which

Nm23 affects tumor metastatic potential have yet to be determined. In murine melanoma cell lines, serine 44 is the major site of

autophosphorylation on Nm23-1. This acid-stable phosphorylation of Nm23 is inhibited in vitro by cAMP and in vivo by forskolin. These data

indicate that this serine phosphorylation is regulated via some cAMP-dependent event in signal transduction. In addition, it has been shown

that the Nm23-H2 protein is identical to the c-myc transcription factor PuF. This suggests that some of the cellular effects of Nm23 are

mediated by its transcriptional regulatory function, while others are mediated by its NDPK activity.

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.