Rap2 Pure 12/Rap2 150ug

Rap2 is a member of the Ras superfamily of low molecular weight GTP/GDP binding proteins. The Rap proteins are 50% homologous in

sequence to p21ras. Like Ras, the Rap proteins cycle between a GDP-bound inactive form and a GTP-bound active form. Conversion between

these two forms is regulated by Rap-GTPase activating protein (Rap-GAP) and a Rap-GDP dissociation stimulator (GDS). Since Ras and Rap

have the same amino acid sequence in their putative effector domain (aa. 32-40), it seems likely that they perform either similar or antagonistic

functions. Rap2 proteins are about 60% identical to Rap1 proteins and Rap2A and Rap2B show 90% amino acid identity, differing mainly at

the carboxy-terminus. Unlike Rap1, the Rap2 proteins cannot compete with Ras for interaction with Ras-GAP, nor are they substrates for

PKA. It follows that the intrinsic GTPase activity of Rap2A is not stimulated by Ras-GAP; however a distinct activator (Rap-GAP) has been

identified. Both Rap2 proteins show posttranslational modifications: Rap2B is geranylgeranylated and Rap2A is the first non-Ras member of

the Ras superfamily observed to be farnesylated.

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.