SAV BV650 100ug

• 产品编号：BD-563855      品牌：BD-Pharmingen       原厂货号：563855
• 产品分类：蛋白质检测与分析 > 蛋白标记和检测 > 亲和素、链霉亲和素、生物素 > 链霉亲和素
• 应用分类：

描述：

Streptavidin is a non-glycosylated protein that is prepared chromatographically from the bacterium Streptomyces avidinii. Streptavidin
homotetramers have a particularly high, non-covalent binding affinity for biotin. When conjugated with fluorochromes, streptavidin has been
widely used with biotin-conjugated antibodies and other biotinylated specific-binding molecules (eg, recombinant proteins and lectins) to stain
cells and tissues for subsequent multiparameter analysis by flow cytometry, fluorescence microscopy and imaging. Likewise, when conjugated
with an enzyme (eg, Horseradish Peroxidase or Alkaline Phosphatase) and coupled with a colorimetric or luminescent substrate development
system, streptavidin has found widespread use along with biotinylated antibodies in a number of applications including Western blot, ELISA,
ELISPOT, immunocytochemistry and immunohistochemistry.
Streptavidin was conjugated to BD Horizon BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem
fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon BV650 can be
excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission
characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor® 700 detectors. However, the spillover can be
corrected through compensation as with any other dye combination.

原厂资料：

注意事项：

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. .
3.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.