Hu Perforin PE-CF594 Dta G9 50Tst

产品编号：BD-563763 品牌：BD-Pharmingen 原厂货号：563763
产品分类：抗体 > 一抗 > 染料标记抗体
应用分类：

包装：

50Tst

运保温度:

Store undiluted at 4°C

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描述：

Perforin has a key role in cell-mediated cytotoxicity. It is a 70 kDa cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic

T lymphocytes (CTLs) and natural killer (NK) cells. CTLs are involved in eliminating virally infected cells, in anti-tumor immune responses,

in allograft rejections, and in some autoimmune diseases. NK cells are important for tumor surveillance and destruction and are involved in

allograft rejections. Cytotoxic cells release the contents of their cytotoxic granules, including perforin upon recognition of their target cell. In

the presence of calcium, perforin forms transmembrane channels or pores in the membrane of the target cell leading to a cell death that

resembles apoptosis. The ability to detect perforin-positive cells with specific antibody should be useful in identifying and understanding

perforin-mediated reactions. Cloneδ

G9 reacts with human and bovine perforin. It does not cross-react with mouse perforin. Purified granules from the human lymphoma

cell line YT were used as immunogen. Cloneδ

G9 was initially characterized by immunoprecipitation and immunohistochemistry of frozen

tissue sections. The antibody stains scattered lymphocytes in red pulp of spleen, and scattered infiltrated lymphocytes in lymphoma.

This antibody is conjugated to BD Horizon™ PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to

PE-Texas Red®. PE-CF594 excites and emits at similar wavelengths to PE-Texas Red® yet exhibits improved brightness and spectral

characteristics. Due to PE having maximal absorption peaks at 496 nm and 564 nm, PE-CF594 can be excited by the blue (488-nm), green

(532-nm) and yellow-green (561-nm) lasers and can be detected with the same filter set as PE-Texas Red® (eg 610/20-nm filter).

注意事项：

1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-μl experimental
sample (a test).
2.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.