描述:
The 9F10 monoclonal antibody specifically reacts with the integrin α
4 chain, that is expressed as a heterodimer with either of two β integrin
subunits, β 1 (CD29) orβ 7. The α4β
1 integrin (VLA-4) is expressed on lymphocytes, monocytes, thymocytes, NK cells, and several B- and T-cell lines, and mediates binding to VCAM-1 (CD106) and the CS-1 region of fibronectin. The α 4 β
7 integrin has a similar tissue distribution,
except it is found on only a small subpopulation of thymocytes. Integrin α 4 β
7 also binds fibronectin and VCAM-1, and has been shown in the
mouse to preferentially bind the mucosal vascular addressin molecule, MAdCAM-1. This antibody is useful for studies of the expression by
and function of cells that express α
4 chain-containing integrins.
This antibody is conjugated to BD Horizon™ PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to
PE-Texas Red®. PE-CF594 excites and emits at similar wavelengths to PE-Texas Red® yet exhibits improved brightness and spectral
characteristics. Due to PE having maximal absorption peaks at 496 nm and 564 nm, PE-CF594 can be excited by the blue (488-nm), green
(532-nm) and yellow-green (561-nm) lasers and can be detected with the same filter set as PE-Texas Red® (eg 610/20-nm filter).