描述：
The Bcl-2/100 monoclonal antibody specifically binds to human Bcl-2. It has been reported not to crossreact with mouse Bcl-2. A synthetic
peptide corresponding to amino acids 41-54 (GAAPAPGIFSSQPG) of human Bcl-2 was used as the immunogen. This peptide sequence
reportedly is not conserved between human and mouse. Programmed cell death (apoptosis) is a normal physiologic process which occurs
during embryonic development as well as in maintenance of tissue homeostasis. The apoptotic program is characterized by certain
morphological features. These include changes in the plasma membrane such as loss of membrane asymmetry and attachment, a condensation
of the cytoplasm and nucleus, and internucleosomal cleavage of DNA. In the final stages, the dying cells become fragmented into “apoptotic
bodies” which are rapidly eliminated by phagocytic cells without eliciting significant inflammatory damage to surrounding cells. Members of
the Bcl-2 family play a major role in regulating the response of cells to apoptotic signals. Bcl-2 is considered to be novel among
proto-oncogenes because it blocks apoptosis in many cell types. Bcl-2 is thought to provide selective survival advantage for cells by blocking
apoptosis and thus may contribute to tumorigenesis. Bcl-2 is an approximately 26 kDa intracellular, integral membrane protein found
primarily in the nuclear envelope, endoplasmic reticulum and outer mitochondrial membrane.
This antibody is conjugated to BD Horizon™ PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to
PE-Texas Red®. PE-CF594 excites and emits at similar wavelengths to PE-Texas Red® yet exhibits improved brightness and spectral
characteristics. Due to PE having maximal absorption peaks at 496 nm and 564 nm, PE-CF594 can be excited by the blue (488-nm), green
(532-nm) and yellow-green (561-nm) lasers and can be detected with the same filter set as PE-Texas Red® (eg 610/20-nm filter).
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