描述:
The CB38 monoclonal antibody specifically binds to CD36. CD36 is a 88 kDa glycoprotein IV (GPIV), the receptor for extracellular matrix
proteins such as collagen and thrombospondin. CD36 is known to mediate the adhesion of
Plasmodium falciparum
. CD36 antigen is
expressed on monocytes, platelets, endothelial cells, and some human tumor cell lines but not on lymphocytes and granulocytes. It is a very
early marker of erythroid differentiation. CD36 antibody induces degranulation, release of ATP and serotonin, increase in [Ca2+]
ι
, and
tyrosine phosphorylation of a substrate protein of 130 kDa.
This antibody is conjugated to BD Horizon BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of
407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD
Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm. Due to the excitation of the
acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon
PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness
equivalent to PE conjugates and can be used as a third color off of the violet laser.
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are
used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon
Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD
Horizon Brilliant Stain Buffer (Cat. No. 563794)