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Ms CD8a BV650 53-6.7 50ug

Ms CD8a BV650 53-6.7 50ug

产品编号：BD-563234 品牌：BD-Pharmingen 原厂货号：563234
产品分类：抗体 > 一抗 > 染料标记抗体
应用分类：

包装：

50ug

运保温度:

Store undiluted at 4°C

到货周期：

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描述：

The 53-6.7 antibody monoclonal antibody specifically binds to the 38 kDaα

and 34 kDa α

' chains of the CD8 differentiation antigen (Ly-2 or

Lyt-2) of all mouse strains tested. The CD8

αandα

' chains (CD8a) form heterodimers with the CD8

chain (CD8b, Ly-3, or Lyt-3) on the

surface of most thymocytes. A subpopulation of mature T lymphocytes (i.e., MHC class I-restricted T cells, including most T

suppressor/cytotoxic cells) expresses almost exclusively the CD8

αβ

heterodimer (the

' chain is absent). Subsets of

γδ

TCR-bearing T cells,

intestinal intrapithelial lymphocytes, and dendritic cells express CD8a without CD8b. It has been suggested that the expression of the

CD8a/CD8b heterodimer is restricted to T lymphocytes which matured in the thymus or in an extrathymic environment that had been

influenced by thymus-initiated neuroendocrine signals. CD8 is an antigen coreceptor on the T-cell surface which interacts with MHC class I

molecules on antigen-presenting cells or epithelial cells. It participates in T-cell activation through its association with the T-cell receptor

complex and protein tyrosine kinase lck (p56 [lck]). The CD8

and

' chains arise from alternatively spliced messengers of a single

CD8a

gene. The longer

form associates with p56 [lck] via a CXCP motif in its cytoplasmic domain, which it shares with CD4, but not with CD8b.

The truncated

' chain is unable to associate with p56 [lck], and it may function to attenuate the CD8-mediated costimulatory signal during

intrathymic T-cell maturation. In vivo and in vitro treatment with 53-6.7 mAb has reportedly been effective at depleting CD8+ peripheral T

lymphocytes. The 53-6.7 antibody has also been reported to cross-reaact with CD8

- and

'-like polypeptides on subsets of thymic and

peripheral lymphocytes in the Egyptian toad,

Bufo regularis

The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. This dye is a

tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon™

BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and

emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor® 700 detectors. However, the spillover can

be corrected through compensation as with any other dye combination.

注意事项：

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. .
3.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.