Erk1/Erk2 (pT202/pY204) BV421 20A 50Tst

产品编号：BD-562981 品牌：BD-Pharmingen 原厂货号：562981
产品分类：抗体 > 一抗 > 磷酸化抗体
应用分类：

包装：

50Tst

运保温度:

Store undiluted at 4°C

到货周期：

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描述：

The members of the Mitogen-Activated Protein Kinase (MAPK) family are components of a key signal transduction cascade that links events
at the cell surface to responses in the nucleus. The signaling cascade is found in species as varied as yeast and humans, with many of the
proteins being well conserved. In mammals the most widely studied members of the cascade are the Extracellular signal-Regulated Kinases,
ERK1 (p44 MAPK) and ERK2 (p42 MAPK). ERK1 and ERK2 share 85% homology and are activated by extracellular signals such as
growth factors, hormones, and phorbol esters. Activation occurs through a series of phosphorylations by kinases activating other kinases and
eventually leading to phosphorylation of the ERKs. Growth factor stimulation leads to activation of Ras and Raf, leading to phosphorylation
of MEK1 (MAPK/ERK kinase) which, in turn, activates the ERKs via dual phosphorylation. Once activated, the ERKs phosphorylate other
cytoplasmic signalling molecules, cell-surface receptors, microtubule-associated proteins, and transcription factors in the nucleus. Thus, the
active ERK has myriad downstream effectors that implicate it in the control of cell proliferation and differentiation, as well as regulation of the
cytoskeleton. Furthermore, studies have shown that elevated ERK activity is associated with some cancers.
The 20A monoclonal antibody recognizes the phosphorylated threonine 202 and tyrosine 204 (pT202/pY204) of human ERK1 and
pT184/pY186 of human ERK2. The orthologous phosphorylation sites in murine ERK1 and ERK2 are T203/Y205 and T183/Y185.
The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. With an Ex Max
of 407-nm and Em Max at 421-nm, BD Horizon™ BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter
set (eg, 450/50-nm filter). BD Horizon™ BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared
to Pacific Blue™ conjugates.

注意事项：

1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-μl experimental
sample (a test).
2.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.