Hu IFN-GMA BV605 B27 50Tst

• 产品编号：BD-562974      品牌：BD-Pharmingen       原厂货号：562974
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

The B27 monoclonal antibody specifically binds to human interferon-γ (IFN-γ). IFN-γ is a potent multifunctional cytokine that is produced
by several activated cell types including NK, NKT, CD4+TCRαβ+, CD8+TCRαβ+, and TCRγδ+ T cells. IFN-γ exerts its biological effects
through specific binding to the high-affinity IFN-γ Receptor Complex comprised of IFN-γRα (CD119) and IFN-γRβ subunits. In addition to
its antiviral effects, IFN-γ upregulates a number of lymphoid cell functions including the antimicrobial and antitumor responses of
macrophages, NK cells, and neutrophils. In addition, IFN-γ can exert strong regulatory influences on the proliferation, differentiation, and
effector responses of B cell and T cell subsets. These influences can involve IFN-γ’s capacity to boost MHC class I and II expression by
antigen-presenting cells as well as to direct effects on B cells and T cells themselves. Human IFN-γ is a 14-18 kDa glycoprotein containing
143 amino acid residues. B27 is a neutralizing antibody. The use of B27 antibody for epitope mapping of human IFN-γ has been described.
The B27 antibody has been reported not to bind to denatured IFN-γ.
This antibody is conjugated to BD Horizon BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of
407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD
Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm. Due to the excitation of the
acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon
PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness
equivalent to PE conjugates and can be used as a third color off of the violet laser.
 
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are
used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon
Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD
Horizon Brilliant Stain Buffer (Cat. No. 563794).1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-μl experimental
sample (a test).
2.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
 

原厂资料：

注意事项：

1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-μl experimental
sample (a test).
2.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.