The BD Pharmingen™ Transcription Factor Buffer Set is optimized for fixing and permeabilizing cells prior to immunofluorescent staining
and flow cytometric analysis of cells that express specific intracytoplasmic and intranuclear proteins. The BD Pharmingen™ Transcription
Factor Buffer Set was designed to improve ease-of-use and minimize processing time, to reduce nonspecific staining, to increase the resolution
of positively stained cells and to significantly reduce cell loss during fixation, permeabilization and staining procedures. Flow cytometric
detection of the many proteins known to be expressed within various intracellular compartments, especially transcription factors, is improved
with BD Pharmingen™ Transcription Factor Buffer Set use. This buffer system has been found useful for fixing and permeabilizing a variety
of cell types from diverse human and mouse tissues. The buffer system is flexible in supporting multiwell-plate high-throughput and bulk
sample analyses and applications that require overnight sample storage. The buffer system has minimal impact on the light-scatter and
autofluorescence characteristics of processed cells resulting in characteristics similar to those observed for freshly prepared, highly viable
primary cells. In many cases the buffer system was found to be compatible with the immunofluorescent staining of cell-surface antigens both
before and after cellular fixation and permeabilization. The buffer system is also compatible with many tandem fluorochromes.