Hu IKK Gma (pS376) PE N19-39 50Tst

• 产品编号：BD-562590      品牌：BD-Pharmingen       原厂货号：562590
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

The N19-39 monoclonal antibody specifically binds to the IKK-gamma protein phosphorylated at the Ser376 site, ie, IKKγ (pS376). IKKγ is also known an
NEMO (NF-kappa-B essential modifier) and is encoded by the IKBKG (inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma) gene. The
nuclear transcription factor kappa-B, NF-κB, is controlled by interaction with an inhibitory subunit, IκB, which restricts NF-κB to the cytoplasm. Following
stimulation by various cytokines or other stimuli, IκB becomes degraded and NF-κB is released to the nucleus. The release of IκB from NF-κB is thought to be
a critical point in the activation of NF-κB signal pathways. Activated NF-κB regulates genes involved in various pathways including inflammation, immunity,
and cell survival signaling pathways. A group of proteins form an NF-κB regulatory complex, or signalsome. Two members of this complex are a pair of closely
related serine/threonine kinases, IKKα and IKKβ (also called IKK-1 and IKK-2), which phosphorylate critical residues of IκB, thus targeting it for subsequent
degradation. The IKK complex contains similar amounts of IKKα, IKKβ as well as two other polypeptides, which are differentially processed forms of a third
subunit, IKKγ. IKKα and IKKβ become activated following phosphorylation by upstream kinases, including NF-κB-inducing kinase (NIK)4 and MEKK1.
IKKβ has been shown to phosphorylate IKKγ at serine 376 in response to signaling through the TNF receptor or the Tax oncoprotein of human T-cell leukemia
virus type 1. IKKγ has an essential regulatory role in the IKK complex and is required for the activation of the NF-κB pathway in response to multiple cellular
stimuli. IKKγ isoforms migrate as a doublet of ~50-55 kDa upon Western blot analysis.
 

原厂资料：

注意事项：

1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 X 10e6 cells in a 100-μl experimental sample (a test).
2.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
5.The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.