SMAD2/3 (pS465/467 PE O72-670 50Tst

• 产品编号：BD-562586      品牌：BD-Pharmingen       原厂货号：562586
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

The O72-670 monoclonal antibody specifically binds to the Smad2 protein phosphorylated at the Ser465/467 sites and the Smad3 protein
phosphorylated at the Ser423/425 sites. Smad2 and Smad3 are members of the Smad superfamily with observed molecular weights of 60 kDa and 52
kDa, respectively. The Smad family consists of three subfamilies: receptor regulated Smads or R-Smads, including Smads1, 2, 3, 5, and 8; common
partner Smad, or Co-Smad, including Smad4; and inhibitory Smads, or I-Smad, including Smads 6 and 7. Activation of TGF-beta superfamily
serine/threonine kinase receptors, such as TGF-beta, activin and BMP receptors, by their bound ligands leads to the phosphorylation of R-Smads at
several sites. It has been shown that the ligand-bound TGF-beta type I receptor directly phosphorylates Ser465 and Ser467 of Smad2 and Ser423 and
Ser425 of Smad3. Phosphorylated R-Smads form complexes with Co-Smad and translocate into the nucleus to regulate transcription affecting a wide
range of critical cellular processes including cell-fate determination, proliferation, morphogenesis, differentiation and apoptosis. The inhibitory Smads
inhibit this pathway through two potential mechanisms: either by preventing R-Smads from binding to their corresponding receptors and/or by
competing with Smad4, the Co-Smad, from binding to R-Smads. High level expression of phosphorylated Smad2 has been associated with poor
prognosis in late stage gastric carcinoma. Roles for Smad2 have been described in thymopoiesis and the TGF-β-mediated induction of regulatory T
cells and Th17 cells. The specificity of the O72-670 mAb was confirmed by Western blot and immunohistochemistry using unconjugated antibody.
 

原厂资料：

注意事项：

1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 X 10e6 cells in a 100-μl experimental sample (a test).
2.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
5.The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.