Transcription Factor Buffer Set 100Tst

• 产品编号：BD-562574      品牌：BD-Pharmingen       原厂货号：562574
• 产品分类：生化试剂 > 免疫分析试剂 > 流式细胞术溶液和试剂
• 应用分类：

其它组分：

描述：

本试剂盒适用于表达特定的胞浆内和胞内蛋白的细胞，在免疫荧光染色和FACS仪分析之前的固定和透性化。本试剂盒的目的，是易于使用和最小化处理时间，以减少非特异性染色，以增加染色细胞的阳性检出率，并显著降低固定、透性化和染色过程中的细胞损失。本试剂盒可提高FACS方法检测细胞器表达多种蛋白质​​特别是转录因子的效率。本缓冲系统有利于多种人和小鼠组织细胞的固定和透性化。本试剂盒使用非常灵活，支持多孔板、高通量分析，以及需要过夜的样品的分析。本缓冲系统可将对光散射和背景荧光的影响降低至最小值。在许多情况下，本缓冲系统可用于固定和透性化之前及之后的细胞表面标志的免疫荧光染色，也可兼用于多种荧光染料。

原厂资料：

The BD Pharmingen™ Transcription Factor Buffer Set is optimized for fixing and permeabilizing cells prior to immunofluorescent staining and flow cytometric analysis of cells that express specific intracytoplasmic and intranuclear proteins. The BD Pharmingen™ Transcription Factor Buffer Set was designed to improve ease-of-use and minimize processing time, to reduce nonspecific staining, to increase the resolution of positively stained cells and to significantly reduce cell loss during fixation, permeabilization and staining procedures. Flow cytometric detection of the many proteins known to be expressed within various intracellular compartments, especially transcription factors, is improved with BD Pharmingen™ Transcription Factor Buffer Set use. This buffer system has been found useful for fixing and permeabilizing a variety of cell types from diverse human and mouse tissues. The buffer system is flexible in supporting multiwell-plate high-throughput and bulk sample analyses and applications that require overnight sample storage. The buffer system has minimal impact on the light-scatter and autofluorescence characteristics of processed cells resulting in characteristics similar to those observed for freshly prepared, highly viable primary cells. In many cases the buffer system was found to be compatible with the immunofluorescent staining of cell-surface antigens both before and after cellular fixation and permeabilization. The buffer system is also compatible with many tandem fluorochromes.

注意事项：

1.Store undiluted at 4°C.

2.Due to the fixation and permeabilization procedure, forward and side light-scatter signals will be slightly different than those of live cells.

3.Target the acquisition for a statistically significant number of events.
4.A titration of the fluorescent antibody's optimal staining amount and optimization of the staining time may be required in your application.