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SSEA-3 PE-CF594 MC-631 50Tst

  • 产品编号:BD-562486      品牌:BD-Pharmingen       原厂货号:562486
  • 产品分类:抗体 > 一抗 > 染料标记抗体
  • 应用分类:
 
包装: 50Tst
运保温度: Store undiluted at 4°C
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描述:

The MC-631 monoclonal antibody reacts with Stage-Specific Embryonic Antigen-3 (SSEA-3), a carbohydrate epitope on the major
ganglioside, but not the neutral glycolipid, of mouse embryos and human teratocarcinoma cells. As its name implies, the expression of
SSEA-3 is stage-specific and can be used to characterize embryonic cells and monitor their differentiation. However, its expression pattern
differs in the human and mouse. In the human, SSEA-3 is found on teratocarcinoma (embryonal carcinoma or EC), embryonic inner cell mass
(ICM), and embryonic stem (ES) cells, and erythrocytes. As human stem cells undergo differentiation, SSEA-3 expression diminishes. In the
mouse, SSEA-3 is found on oocytes, ova, zygotes, early cleavage-stage embryos, early blastocysts, ICM, primitive endoderm, and adult
kidney, but not on EC or ES cells.
This antibody is conjugated to BD Horizon™ PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to
PE-Texas Red®. PE-CF594 excites and emits at similar wavelengths to PE-Texas Red® yet exhibits improved brightness and spectral
characteristics. Due to PE having maximal absorption peaks at 496 nm and 564 nm, PE-CF594 can be excited by the blue (488-nm), green
(532-nm) and yellow-green (561-nm) lasers and can be detected with the same filter set as PE-Texas Red® (eg 610/20-nm filter).


注意事项:

1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-μl experimental
sample (a test).
2.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.


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