JNK (PT183/PY185) Alexa 647 N9-66 50Tst

产品编号：BD-562481 品牌：BD-Pharmingen 原厂货号：562481
产品分类：抗体 > 一抗 > 染料标记抗体
应用分类：

包装：

50Tst

运保温度:

Store undiluted at 4°C

到货周期：

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描述：

The N9-66 monoclonal antibody specifically binds to JNK1 and JNK2 phosphorylated at the pT183/pY185 sites. c-Jun NH2-terminal Kinases
(JNKs), also called Stress Activated Protein Kinases (SAPKs), are mitogen-activated protein kinases (MAPKs) with observed molecular
weights of ~46 kDa (JNK1) and ~54 kDa (JNK2). Along with the p38 and ERK families, JNK represents one of three major classes of
MAPKs. Complete activation of JNK requires the phosphorylation of both Thr183 and Tyr185 that are located in a Thr-X-Tyr motif.
Phosphorylation of these residues is carried out by MKK4 and MKK7 that are phosphorylated and activated by MEKKs and MLKs in
response to stress signals delivered through small GTPases of the Rho family. Once activated, JNK can translocate into the nucleus and
regulate the expression of genes through phosphorylation of c-Jun, ATF-2, and other transcription factors. JNK plays a role in signal
transduction in response to cytokines and various forms of environmental stress, such as endotoxins, UV irradiation, heat, and
hyperosmolarity. JNK is critical to the regulation of cell growth, apoptosis, and the cellular response to stress, making it an important factor in
tumorigenesis and adaptive immunity. During antibody development, the N9-66 monoclonal antibody was found to detect phosphorylated
JNK1/2 by Western blot analysis of cellular lysates and by immunofluorescent staining and flow cytometric analysis of fixed and
permeabilized cells. This antibody crossreacts with phosphorylated JNK1/2 expressed by mouse cells, as tested by Western blot analysis and
flow cytometry.

注意事项：

1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-μl experimental
sample (a test).
2.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.