AKT (PS473) PE-CF594 M89-61 50Tst

产品编号：BD-562465 品牌：BD-Pharmingen 原厂货号：562465
产品分类：抗体 > 一抗 > 磷酸化抗体
应用分类：

包装：

50Tst

运保温度:

Store undiluted at 4°C

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描述：

Akt [also known as PKB (Protein kinase B) or RAC-PK (Related to the A and C kinases)] is a family of serine/threonine kinases that contains
a pleckstrin homology (PH) domain. PH domains play important roles in signal transduction. There are three known isoforms of Akt in
mammalian cells [Akt1 (α), Akt2 (β) and Akt3 (γ)]; they are thought to be regulated similarly. Akt is activated by insulin and growth factors
by a mechanism involving phosphoinositide 3-OH kinase. Phosphoinositide 3-OH kinases products bind to the PH domain, resulting in
translocation of Akt to the plasma membrane and activation of Akt to phospho-Akt by upstream kinases. Akt is phosphorylated within the
activation loop at threonine 308 and the C-terminus at serine 473 (S473). Phospho-Akt promotes cell survival by inhibiting apoptosis.
Specifically, phospho-Akt1 has been shown to phosphorylate Bad, a member of the Bcl-2 family that promotes cell death. This
phosphorylation results in the inactivation of the proapoptotic function of Bad. The Akt molecule is thus considered to link extracellular
survival signals (growth factors) with the apoptotic machinery (BAD). Akt is also a key mediator of the metabolic effects of insulin.
Additionally, Akt has been referred to as an oncogene because it has increased activity in a number of tumors.
The M89-61 antibody recognizes Akt phosphorylated at S473. This phosphorylation site is shared by all three isoforms of Akt. The
homologous phosphorylation sites in Akt2 and Akt3 are S474 and S472, respectively.
This antibody is conjugated to BD Horizon™ PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to
PE-Texas Red®. PE-CF594 excites and emits at similar wavelengths to PE-Texas Red® yet exhibits improved brightness and spectral
characteristics. Due to PE having maximal absorption peaks at 496 nm and 564 nm, PE-CF594 can be excited by the blue (488-nm), green
(532-nm) and yellow-green (561-nm) lasers and can be detected with the same filter set as PE-Texas Red® (eg 610/20-nm filter).

注意事项：

1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-μl experimental
sample (a test).
2.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.