Streptavidin PE-CF594 25ug

• 产品编号：BD-562318      品牌：BD-Pharmingen       原厂货号：562318
• 产品分类：蛋白质检测与分析 > 蛋白标记和检测 > 亲和素、链霉亲和素、生物素 > 链霉亲和素
• 应用分类：

原厂资料：

Streptavidin is a non-glycosylated protein that is prepared chromatographically from the bacterium Streptomyces avidinii. Streptavidin homotetramers have a particularly high, non-covalent binding affinity for biotin. When conjugated with fluorochromes, streptavidin has been widely used with biotin-conjugated antibodies and other biotinylated specific-binding molecules (eg, recombinant proteins and lectins) to stain

cells and tissues for subsequent multiparameter analysis by flow cytometry, fluorescence microscopy and imaging. Likewise, when conjugated with an enzyme (eg, Horseradish Peroxidase or Alkaline Phosphatase) and coupled with a colorimetric or luminescent substrate development system, streptavidin has found widespread use along with biotinylated antibodies in a number of applications including Western blot, ELISA,

BD™ ELISPOT, immunocytochemistry and immunohistochemistry.

Streptavidin is conjugated to BD Horizon PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to PE-Texas Red?. PE-CF594 excites and emits at similar wavelengths to PE-Texas Red? yet exhibits improved brightness and spectral characteristics. Due to PE having maximal absorption peaks at 496 nm and 564 nm, PE-CF594 can be excited by the blue (488-nm), green(532-nm) and yellow-green (561-nm) lasers and can be detected with the same filter set as PE-Texas Red? (eg 610/20-nm filter).

注意事项：

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. When excited by the yellow-green (561-nm) laser, the fluorescence may be brighter than when excited by the blue (488-nm) laser.

3.Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.

4.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
 

3.