Fixable Viability Stain 450 100ug

• 产品编号：BD-562247      品牌：BD-Pharmingen       原厂货号：562247
• 产品分类：生化和细胞分析 > 细胞分析试剂盒 > 细胞活性及活力检测试剂盒
• 应用分类：

描述：

BD Horizon™ Fixable Viability Stain 450 (FVS450) is useful to discriminate viable from non-viable mammalian cells in multicolor flow
cytometric applications. This violet fluorescent stain contains a dye that reacts with and covalently binds to cell surface and intracellular
amines. Permeable plasma cell membranes, such as those present in necrotic cells, allow for the intracellular diffusion of the violet dye and
covalent binding to higher overall concentrations of amines than in non-permeable live cells. Therefore, necrotic cells present in a typical in
vitro assay label with higher levels of dye increasing their fluorescence intensity 10-20 fold over that of viable cells. The labeled cells can be
fixed with formaldehyde for downstream decontamination, freezing and/or permeablization and subsequent intracellular staining while
maintaining stable FVS450 fluorescence.
The BD Horizon™ Fixable Viability Stain 450 is excited by the Violet laser (with an excitation maximum of 406 nm) and has a fluorescence
emission maximum at 450 nm.

原厂资料：

注意事项：

Upon arrival, store the dry dye at -80°C until use. After reconstitution with DMSO, store the solution at -20°C. The dye solution can be used for
up to four freeze-thaw cycles. Aliquots (eg, ~100 μl aliquots) can be made and stored at -20°C when required for smaller experiments. Do not use
reconstituted dye after 40 days of storage. Please discard the dye solution after 40 days post reconstitution with DMSO.