Ms CD62L PE MEL-14 25ug

• 产品编号：BD-561918      品牌：BD-Pharmingen       原厂货号：561918
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

原厂资料：

The MEL-14 antibody reacts with CD62L (L-selectin), a 95 kDa (on neutrophils) or 74 kDa (on lymphocytes) receptor with lectin-like and
Epidermal Growth Factor-like domains. In the mouse, L-selectin is detected on most thymocytes, with the highest levels of expression on an
immunocompetent subset and a population of dividing progenitor cells, and on peripheral leukocytes, including subsets of B and T
lymphocytes, neutrophils, monocytes, and eosinophils. This member of the selectin adhesion molecule family appears to be required for
lymphocyte homing to peripheral lymph nodes and to contribute to neutrophil emigration at inflammatory sites. L-selectin is rapidly shed from
lymphocytes and neutrophils upon cell activation, metalloproteinases may mediate the release of CD62L ectodomains from the cell surface.
The level of CD62L expression, along with other markers, distinguishes naive, effector, and memory T cells. L-selectin binds to sialytaed
oligosaccharide determinants on high endothelial venules (HEV) in peripheral lymph nodes. In vitro studies have demonstrated that CD34,
GlyCAM-1, and MAdCAM-1, all recognized by mAb MECA-79 (anti-mouse PNAd Carbohydrate Epitope, Cat. No. 553863), may be ligands
for CD62L. MEL-14 mAb blocks in vitro binding of lymphocytes to peripheral lymph node HEV and inhibits in vivo lymphocyte
extravasation into peripheral lymph nodes and late stages of leukocyte rolling.

注意事项：

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
4.The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
5.The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.