HOECHST 33342 Soln 1mg

• 产品编号：BD-561908      品牌：BD-Pharmingen       原厂货号：561908
• 产品分类：生化试剂 > 染料/染色剂/指示剂/底物 > 细胞染料 > 细胞核酸染料
• 应用分类：核酸分离纯化与分析 > 核酸凝胶电泳与印迹 > DNA染料

描述：

The BD Pharmingen™ Hoechst 33342 Solution is a reagent for the fluorescent staining of DNA and nuclei in live or fixed cells. Hoechst
33342 is a bisbenzimidazole dye with high specificity for binding to double-stranded DNA (preferentially binds to A-T base pairs). This dye
is very useful to label double-stranded DNA and thus to visualize nuclei. Hoechst 33342 can be excited at ~355 nm by a UV light source (eg,
UV laser beam or a mercury arc-lamp). It emits blue fluorescence light around an emission maximum at 461 nm when bound to DNA. Since
the Hoechst 33342 dye is specific for DNA binding, ribonuclease treatment is not needed to avoid nonspecific RNA staining. In addition to its
use in fluorescence microscopy and image analysis, Hoechst 33342 is commonly used for flow cytometric applications, such as cell cycle
analysis and stem cell side population identification.
 

原厂资料：

注意事项：

1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-μl experimental
sample (a test).
2.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.