Hu Naive/MEM T Cell Panel 50Tst

产品编号：BD-561438 品牌：BD-Pharmingen 原厂货号：561438
产品分类：抗体 > 一抗 > 复合抗体试剂盒
应用分类：

包装：

50Tst

运保温度:

Store undiluted at 4°C

到货周期：

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描述：

Multicolor immunofluorescent staining followed by flow cytometric analysis and sorting has led to the phenotypic and functional
characterization of multiple peripheral T cell subsets. Three major T cell subsets have been well characterized, i.e., naïve, memory and effector
T cells. Naïve T cells have a relatively homogenous cell surface phenotype. They express high levels of CD45RA and the CD197 (CCR7
chemokine receptor). Effector T cells coexpress variable to low levels of CD45RA and no CD197 whereas memory T cells coexpress variable
561438 Rev. 3 Page 1 of 3to low levels of CD45RA and high levels of CD197. The Human Naive/Memory T Cell Panel contains fluorescent antibodies (each optimized
at 5 μl per test) that are specific for the cell surface antigens: CD45RA, CD197, CD4 and CD3. The channel for detecting Phycoerythrin or
Phycoerythrin protein-tandem conjugated proteins has been left open for inclusion of other markers. The panel was designed to standardize the
multicolor staining and flow cytometric characterization of the three major CD4+ T cell subsets that arise as a consequence of development or
clonal expansion and differentiation driven by antigenic stimulation (eg, in response to allergens, infectious disease or vaccination).

注意事项：

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
The antibody was conjugated with APC-H7 under optimum conditions, and unconjugated antibody and APC-H7 were removed.
The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were
removed. Storage of PerCP-Cy5.5 conjugates in unoptimized diluent is not recommended and may result in loss of signal intensity.
The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.