Hu CD27 Horizon V500 M-T271 25Tst

产品编号：BD-561223 品牌：BD-Pharmingen 原厂货号：561223
产品分类：抗体 > 一抗 > 染料标记抗体
应用分类：

包装：

25Tst

运保温度:

Store undiluted at 4°C

到货周期：

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描述：

The M-T271 monoclonal antibody specifically reacts with CD27. CD27 presents as a type I transmembrane, disulphide-linked 110 kDa

homodimer comprised of two polypeptide chains. The CD27 molecule is a lymphocyte-specific member of the TNF/NGF-R family, and is

expressed on a subset of human thymocytes and on the majority of mature T lymphocytes, activated B cells and NK cells. CD27 is highly

induced on T cells after TCR stimulation. CD27 binds to CD70 (also known as, CD27 ligand or CD27L) and may be involved in cellular

interaction of T and B lymphocytes.

The antibody is conjugated to BD Horizon™ V500, which has been developed for use in multicolor flow cytometry experiments and is

available exclusively from BD Biosciences. It is excited by the Violet laser with an Ex max of 415 nm and Em Max at 500 nm. BD Horizon

V500 conjugates emit at a similar wavelength to Amcyan yet exhibit reduced spillover into the FITC channel. For more information on BD

Horizon V500, visit bdbiosciences.com/colors.

When compensating dyes in this spectral range (such as Horizon™ V500 and AmCyan), the most accurate compensation can be obtained using

single stained cellular controls. Due to spectral differences between cells and beads in this channel, using BD CompBeads can result in

spillover errors for V500 and AmCyan reagents. Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values

for these reagents is not recommended. Different V500 reagents (e.g. CD4 vs. CD45) can have slightly different fluorescence spillover

therefore, it may also be necessary to use clone specific compensation controls when using these reagents.

注意事项：

1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-μl experimental
sample (a test).
2.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.