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BrdU PerCP-Cy5.5 3D4 50Tst

  • 产品编号:BD-560809      品牌:BD-Pharmingen       原厂货号:560809
  • 产品分类:抗体 > 一抗 > 染料标记抗体
  • 应用分类:
 
包装: 50Tst
运保温度: Store undiluted at 4°C
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描述:

Bromodeoxyuridine (BrdU) is an analog of thymidine that can be incorporated into newly synthesized DNA by cells entering and progressing
thru the DNA synthesis (S) phase of the cell cycle. The amount of BrdU that gets incorporated is dependent upon the amount of time that the
cells are exposed to BrdU (pulse time), the rate of cell division, and whether the cells are in early, mid, or late S phase. Detection of
incorporated BrdU allows the investigator to identify cycling cells in an asynchronous cell population and to determine cell cycle kinetics.
The 3D4 monoclonal antibody reacts with BrdU, but not other nucleotides, in single-stranded DNA. Random cleavage (nicking) of cellular
DNA with DNase I permits the binding of the antibody to incorporated BrdU.


注意事项:

1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-μl experimental
sample (a test).
2.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
 


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