Ms TH17/TREG Phenotyping Kit 50Tst

• 产品编号：BD-560767      品牌：BD-Pharmingen       原厂货号：560767
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

描述：

The immune system protects individuals from a broad range of pathogenic microorganisms while avoiding inappropriate or extreme immune
responses, such as autoimmune responses that could be harmful. The peripheral CD4+ T cell pool includes multiple functionally-distinct T
cell subsets that arise through thymic differentiation or as a consequence of antigen-driven expansion and differentiation of peripheral na飗e T
cells. The early response of na飗e CD4+ T cells to antigenic stimulation may be characterized by high level proliferation and a limited cytokine
repertoire. Further differentiation yields cells with a more diverse potential for cytokine expression. Depending upon the balance of local
cytokines, costimulatory molecules, antigen levels, and genetic factors, Th17 effector/memory cells or inducible CD4+ T regulatory cells
(iTregs) can be generated from the na飗e CD4+ T cell pool. In addition, the peripheral T cell pool contains natural CD4+ T regulatory cells
(nTregs) that are generated in the thymus as a functionally mature subpopulation of T cells.
Functionally-polarized CD4+ T cell subsets have been identified based on their distinctive patterns of cytokine secretion, transcription factor
expression and function. As a signature cytokine, Th17 express high levels of interleukin-17A (IL-17A) whereas Treg are characterized by the
expression of the FoxP3 transcription factor. Through the secretion of IL-17A and other factors, Th17 cells recruit and activate neutrophils
and mediate immune responses against extracellular bacteria and fungi. Th17 cells are also implicated in mediating autoimmune responses.
Natural and inducible Tregs can suppress the function of other T cells or cell types involved in the immune response through a variety of
mechanisms including cytokines. In this way, Treg safeguard against the immune system's responsiveness to self antigens and restrain
excessive responsiveness to foreign antigens that could be harmful to the host.
The Th17/Treg paradigm provides a useful model system for investigating the cellular and molecular mechanisms that mediate protective as
well as harmful immune responses including autoimmune diseases. The BD Mouse Th17/Treg Phenotyping Kit provides an easy-to-use
three-color cocktail of fluorescent antibodies-specific for mouse CD4, IL-17A (for Th17) and Foxp3 (for Treg)-that will enable researchers to
identify and characterize the nature of the CD4+ T cell types present in their system by multicolor flow cytometric analysis. The kit can be
used to successfully analyze ex vivo lymphoid cell samples (eg, for the types of in vivo-generated mouse CD4+ T cell subsets) or to monitor
CD4+ T cell differentiation of cells cultured within various experimental model systems.
Investigators should note that the appearance of BD GolgiStop?Protein Transport Inhibitor may range in color from clear (colorless) to light
yellow.
560767

原厂资料：

注意事项：

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were
removed. Storage of PerCP-Cy5.5 conjugates in unoptimized diluent is not recommended and may result in loss of signal intensity.
The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.