Hu TH17/Treg Phenotyping Kit 50Tst

• 产品编号：BD-560762      品牌：BD-Pharmingen       原厂货号：560762
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

描述：

The immune system protects individuals from a broad range of pathogenic microorganisms while avoiding inappropriate or
extreme immune responses, such as autoimmune responses that could be harmful. The peripheral CD4+ T cell pool includes
multiple functionally-distinct T cell subsets that arise through thymic differentiation or as a consequence of antigen-driven
expansion and differentiation of peripheral naïve T cells. The early response of naïve CD4+ T cells to antigenic stimulation may
be characterized by high level proliferation and a limited cytokine repertoire. Further differentiation yields cells with a more
diverse potential for cytokine expression. Depending upon the balance of local cytokines, costimulatory molecules, antigen
levels, and genetic factors, Th17 effector/memory cells or inducible CD4+ T regulatory cells (iTreg) can be generated from the
naïve CD4+ T cell pool. In addition, the peripheral T cell pool contains natural CD4+ T regulatory cells (nTreg) that are
generated in the thymus as a functionally mature subpopulation of T cells.
Functionally-polarized CD4+ T cell subsets have been identified based on their distinctive patterns of cytokine secretion,
transcription factor expression and function. As a signature cytokine, Th17 express high levels of interleukin-17A (IL-17A)
whereas Treg are characterized by the expression of the FoxP3 transcription factor. Through the secretion of IL-17A and other
factors, Th17 cells recruit and activate neutrophils and mediate immune responses against extracellular bacteria and fungi. Th17
cells are also implicated in mediating autoimmune responses. Natural and inducible Tregs can suppress the function of other T
cells or cell types involved in the immune response through a variety of mechanisms including cytokines. In this way, Treg
safeguard against the immune system’s responsiveness to self antigens and restrain excessive responsiveness to foreign antigens
that could be harmful to the host.
The Th17/Treg paradigm provides a useful model system for investigating the cellular and molecular mechanisms that mediate
protective as well as harmful immune responses including autoimmune diseases. The BD Human Th17/Treg Phenotyping Kit
provides an easy-to-use three-color cocktail of fluorescent antibodies-specific for human CD4, IL-17A (for Th17) and Foxp3
(for Treg)-that will enable researchers to identify and characterize the nature of the CD4+ T cell types present in their system by
multicolor flow cytometric analysis. The kit can be used to successfully analyze ex vivo lymphoid cell samples (eg, for the types
of in vivo-generated human peripheral blood CD4+ T cells) or to monitor CD4+ T cell differentiation by cells cultured within
various experimental model systems.
Investigators should note that the appearance of BD GolgiStop™ Protein Transport Inhibitor may range in color from clear (colorless) to light
yellow.

原厂资料：

注意事项：

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were
removed. Storage of PerCP-Cy5.5 conjugates in unoptimized diluent is not recommended and may result in loss of signal intensity.