Hu T Cell Kit Lyo Cells

• 产品编号：BD-560760      品牌：BD-Pharmingen       原厂货号：560760
• 产品分类：细胞,细胞培养与转染试剂 > 细胞 > 哺乳动物细胞系
• 应用分类：

描述：

The BD Phosflow™ T Cell Kit Lyophilized Cells contains five vials each of lyophilized Treated and Untreated Human Control Cells. Each vial of
control cells contains fixed, permeabilized, and lyophilized human peripheral blood leukocytes (PBL). The cells are intended to serve as biological
controls for antibody staining in BD Phosflow studies designed to analyze cell signaling events associated with T-cell activation. These events include
the phosphorylation status of signaling molecules that mediate the intracellular MAPK and JAK/STAT signaling pathway responses transduced by
stimulated human CD4+ and CD8+ T cells.
The BD Phosflow™ Treated Human Control Cells - T cells are PBL that were stimulated to express easily detectable intracellular levels of several
phosphorylated signaling molecules including the serine/threonine kinases, Erk1/2 (p44/p42 MAPK) and p38 MAPK and Signal Transducers and
Activators of Transcription (STAT) proteins, Stat1, Stat3, Stat5, and Stat6 as determined by multicolor BD Phosflow™ analysis. The treated control
cells were activated with phorbol 12-myristate 13-acetate (PMA), interferon-alpha (IFN-α), interleukin-2 (IL-2), IL-4, and IL-6. These activators are
known to induce the cellular phosphorylation of the specified molecules that are often targeted in T cell signaling studies. Each vial of BD Phosflow™
Untreated Human Control Cells - T cells contains PBL from the same donor as the treated cells. These control cells were cultured without activators
and express background levels of the phosphorylated target signal proteins. The Treated and Untreated Control Cells were fixed with BD Phosflow™
Lyse/Fix Buffer (Cat. No. 558049) and permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050). The cells were then washed with BD
Pharmingen™ Stain Buffer (FBS) (Cat. No. 554656) and lyophilized. Each vial contains a sufficient number of Treated or Untreated Control Cells for
one multicolor stain using fluorescent antibodies specific for CD3, CD4, and CD8 and a single phosphorylated target protein.
Each lot of Treated Control Cells contains a measurable proportion of cells that express the specified phosphorylated signaling proteins.
Representative BD Phosflow results are shown for typical staining of CD4+ and CD8+ Treated and Untreated Control Cells. Data from individual lots
of these control cells may differ due to donor variation. Investigators should anticipate similar (though not identical) results to those shown due to
differences in staining methodology, fluorescent antibody reagents and flow cytometers or cytometer settings.

原厂资料：

注意事项：

Store unopened vials of lyophilized cells at 2 - 8°C. Do not freeze. Vials should be opened and the lyophilized cells reconstituted just
prior to use.