Hu TH1/17 Phenotyping Kit 50Tst

• 产品编号：BD-560752      品牌：BD-Pharmingen       原厂货号：560752
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

描述：

The peripheral CD4+ T cell pool includes multiple effector and memory T cell subsets that arise through antigen-driven expansion and
differentiation of naïve T cells. The early response of naïve CD4+ T cells to antigenic stimulation is characterized by high level proliferation
and a limited cytokine repertoire. Further differentiation yields cells with a more diverse potential for cytokine expression. Depending upon
the balance of local cytokines, costimulatory molecules, antigen levels, and genetic factors, Type-1 T helper (Th1) and Th17 effector and/or
memory cells are generated by immune responses.
Functionally-polarized CD4+ T cell subsets have been identified based on their distinctive patterns of cytokine secretion. As a signature
cytokine, Th1 cells selectively produce large amounts of interferon-gamma (IFN-γ) whereas Th17 express high levels of IL-17A. Through
secretion of IFN-γ and other effector molecules, Th1 cells activate macrophages, natural killer (NK) cells, and CD8+ T cells and are
responsible for cell-mediated immunity. Th1 cells provide protection against intracellular bacteria, fungi, protozoa and viruses and are
involved in some autoimmune responses. By the secretion of IL-17A and other factors, Th17 cells recruit and activate neutrophils and mediate
immune responses against extracellular bacteria and fungi. Th17 cells are also implicated in autoimmune responses. In addition to these types
of T helper cells, Th17/Th1-like (IL-17A/IFN-γ) cells that coexpress both signature cytokines have been described.
The Th1/Th17 paradigm provides a useful model for investigating the cellular and molecular mechanisms that mediate protective as well as
harmful immune responses. The BD Human Th1/Th17 Phenotyping Kit provides an easy-to-use three-color cocktail of fluorescent
antibodies-specific for human CD4, IFN-γ (for Th1) and IL-17A (for Th17)-that will enable researchers to identify and characterize the nature
of these T helper cell types by multicolor flow cytometric analysis. The kit can be used to successfully analyze ex vivo lymphoid cell samples
(eg, for the types of in vivo-generated human peripheral blood T helper cells) or to monitor T helper cell differentiation by cells cultured
within various experimental model systems.
Investigators should note that the appearance of BD GolgiStop™ Protein Transport Inhibitor may range in color from clear (colorless) to light
yellow.
 

原厂资料：

注意事项：

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. .
3.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.