Ms IL-4 PE-Cy7 11B11 50ug

• 产品编号：BD-560699      品牌：BD-Pharmingen       原厂货号：560699
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

原厂资料：

The 11B11 antibody reacts with mouse interleukin-4 (IL-4). The immunogen used to generate the 11B11 hybridoma was partially purified mouse IL-4 from PMA-stimulated EL-4 supernatant. The purified or unconjugated form of this antibody has been reported to be neutralizing.

注意事项：

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.PE-Cy7 is a tandem fluorochrome composed of R-phycoerythrin (PE), which is excited by 488-nm light and serves as an energy donor,coupled to the cyanine dye Cy7, which acts as an energy acceptor and fluoresces maximally at 780 nm. PE-Cy7 tandem fluorochrome
emission is collected in a detector for fluorescence wavelengths of 750 nm and higher. Although every effort is made to minimize the lot-to-lot variation in the efficiency of the fluorochrome energy transfer, differences in the residual emission from PE may be observed.
Therefore, we recommend that individual compensation controls be performed for every PE-Cy7 conjugate. PE-Cy7 is optimized for use with a single argon ion laser emitting 488-nm light, and there is no significant overlap between PE-Cy7 and FITC emission spectra. When
using dual-laser cytometers, which may directly excite both PE and Cy7, we recommend the use of cross-beam compensation during data acquisition or software compensation during data analysis.
3.Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum
foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
4.The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
5.The antibody was conjugated with PE-Cy7 under optimum conditions, and unconjugated antibody and free PE-Cy7 were removed.
6.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.