Histone H3(pS28) HorizonV450 HTA28 50Tst

• 产品编号：BD-560606      品牌：BD-Pharmingen       原厂货号：560606
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

Histones are highly basic proteins that complex with DNA to form chromatin. Histone H3 is a ~15-kDa protein that is phosphorylated at
serine 28 (S28), S10, and/or threonine 11 during mammalian cell mitosis and meiosis. The phosphorylation sites are located in the N-terminal
tail, a region that is outside of the chromatin fiber and is thus accessible for interactions with agents that may regulate chromatin or specific
gene activities. The phosphorylation states of the two serine sites during the cell cycle are highly regulated by Aurora B kinase and a PP1
phosphatase: S10 is in the phosphorylated state from late G2 phase to anaphase, while S28 is phosphorylated from prophase to anaphase.
Furthermore, phosphorylation of histone H3 S28 may be mediated by other kinases in response to external stimuli. Evidence suggests that
histone phosphorylation is involved in the regulation of chromosome condensation, cell division, and gene transcription.
The HTA28 monoclonal antibody reacts with histone H3 phosphorylated at S28 in its N-terminal tail. It does not recognize the
unphosphorylated protein.
HTA28 has been predicted to be reactive on human, rat, hamster, and cow samples. Investigators are advised that the BD Hoirzon™ V450
Rat Anti-Histone H3 (pS28) antibody (clone HTA28) is not routinely tested on human, rat, hamster, and cow samples.
The antibody is conjugated to BD Horizon™ V450, which has been developed for use in multicolor flow cytometry experiments and is
available exclusively from BD Biosciences. It is excited by the Violet laser Ex max of 406 nm and has an Em Max at 450 nm. Conjugates with
BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates.

原厂资料：

注意事项：

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of

potentially explosive deposits in plumbing. .
3.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.