SHP2 (pY542) Alexa 647 L99-921 50Tst

• 产品编号：BD-560390      品牌：BD-Pharmingen       原厂货号：560390
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

SHP2 is a member of the cytosolic class of protein-tyrosine phosphatases (PTPs). SHP2 reportedly contains two SH2 domains, both of which
are N-terminal to the PTP catalytic domain. SH2 PTPs are believed to work in conjunction with protein-tyrosine kinases to maintain
intracellular protein phosphotyrosine homeostasis and cell cycle progression. The expression of SHP2 has been reported to be highest in brain,
heart, and kidney. SHP2 is phosphorylated at two C-terminal tyrosine residues: Y542 and Y580. Phosphorylation of Y542 creates an
interaction in the N-terminal SH2 domain and appears to relieve basal inhibition of the PTP, while phosphorylation at Y580 creates an
interaction at the C-terminal that stimulates PTP activity.
The L99-921 monoclonal antibody recognizes the phosphorylated Y542 of SHP2 (PTP2C isoform 2). The homologous phosphorylation site in
the PTP2Ci splice variant (isoform 1) is Y546. The specificity of this antibody was validated by confirming, using western blot analysis, that
RNA-mediated interference (RNAi) of the specific protein was able to down-regulate the expression of SHP2 (pY542).

原厂资料：

注意事项：

1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-μl experimental
sample (a test).
2.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3.Store undiluted at 4°Cand protected from prolonged exposure to light. Do not freeze.