Hu IRAK4 Alexa 647 L29-525 50Tst

• 产品编号：BD-560315      品牌：BD-Pharmingen       原厂货号：560315
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

Interleukin 1 (IL-1) is a proinflammatory cytokine that mediates the host inflammatory response. IL-1 exerts its biological effects through its
type I receptor (IL-1RI), which is found on most cell types. When IL-1 binds to the IL-1RI, NF-κB (transcription factor) is activated and
translocates to the nucleus where it induces gene expression. IL-1-receptor-associated kinase (IRAK) has been reported to
co-immunoprecipitate with IL-1RI. The IRAK family is made up of four identified Pelle homologs that regulate the activation of NF-κB and
mitogen-activated protein (MAP) kinase pathways. IRAK4 is a unique member of the IRAK family because it is the closest mammalian
homolog to Pelle. Also, when overexpressed, its kinase activity allows it to activate both the NF-κB and mitogen-activated protein (MAP)
kinase pathways, and block the IL-1-induced activation and modification of IRAK1. IRAK4 is also able to phosphorylate IRAK1, which
suggests that IRAK4 affects the early signal transduction of Toll/IL-1 receptors before the involvement of IRAK1.
The L29-525 monoclonal antibody recognizes human IRAK4, regardless of phosphorylation status. The specificity of this antibody conjugate
for flow cytometric analysis was validated by confirming that RNA-mediated interference (RNAi) of the specific protein reduced the staining
of the cells (see figure). Furthermore, the capacity of the RNAi to down-regulate the expression of the relevant protein was confirmed by
western blot analysis.

原厂资料：

注意事项：

1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-μl experimental
sample (a test).
2.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.