Stat1 (pY701) Pac Blue 14/P-STAT1 50Tst

• 产品编号：BD-560310      品牌：BD-Pharmingen       原厂货号：560310
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

Stat (Signal transducer and activators of transcription) proteins are critical mediators of the biologic activity of cytokines, including
interleukins, interferons, erythropoietin, and growth factors. Ligand-receptor interaction leads to activation of constitutively associated JAK
family kinases and subsequent recruitment/activation of Stat proteins by tyrosine phosphorylation. Active Stat proteins then move to the
nucleus to promote transcription of cytokine-inducible genes. Seven Stat proteins have been cloned, each of which is differentially expressed
and/or activated in a cytokine-specific and cell type-specific manner. Stat1 and Stat2 are components of the ISGF3 (Interferon-Stimulated
Gene Factor 3) complex, which is the primary transcription activator induced by the binding of the interferon to a specific cell-surface
receptor. Stat1 has two alternatively spliced isoforms, 91-kDa Stat1α and 84-kDa Stat1β; Stat1α has 38 additional C-terminal amino acids. In
response to the binding of IFNα, IFNγ, EGF, PDGF, or CSF-1 to their respective receptors, the Stat1 subunits become
tyrosine-phosphorylated at Y701, and the complex is translocated to the nucleus. This results in the formation of an active complex that
includes the DNA-binding p48 subunit. This complex is responsible for modulating the transcription of the interferon-stimulated genes
(ISGs).
The 14/P-STAT1 monoclonal antibody recognizes the phosphorylated Y701 in Stat1α and Stat1β.

原厂资料：

注意事项：

1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-μl experimental
sample (a test).
2.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.