PKC-Theta PE 27 50Tst

• 产品编号：BD-560216      品牌：BD-Pharmingen       原厂货号：560216
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes, such as growth,
differentiation, and cytokine secretion. At least eleven isozymes have been described. These proteins are products of multiple genes and
alternative splicing. Conventional PKC (cPKC) subfamily members (a, b, and γ isoforms) consists of a single polypeptide chain containing
four conserved regions (C) and five variable regions (V). The N-terminal half containing C1, C2, V1, and V2 constitutes the regulatory
domain and interacts with the PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester. However, the the C2-like domains of
novel PKC (nPKC) subfamily members (δ, ε, η, and θ isoforms) are Ca2+-independent. The atypical PKC (aPKC) subfamily members (ζ, ι,
and λ isoforms) lack the C2 domain and are unique in that their activity is independent of diacylglycerols and phorbol esters. They also lack
one repeat of the cysteine-rich sequences that are conserved in cPKC and nPKC members. The C-terminal region of PKC contains the
catalytic domain. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of
transmembrane receptors by hormones, neurotransmitters, and growth factors. PKCθ transcripts are expressed in most tissues with the highest
levels being found in hematopoietic tissues and cell lines, including T cells and thymocytes. PKCθ mRNA is readily detectable in skeletal
muscle, lung and brain. However, PKCθ expression is not detected in several human carcinoma cell lines. Abundant expression of this PKC
isozyme in hematopoietic cells suggest that it may have a role in growth and differentiation processes of these cells.

原厂资料：

注意事项：

1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 X 10e6 cells in a 100-μl experimental sample (a test).
2.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
5.The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.