PKA RII Alp (pS99) Alexa 647 50Tst

• 产品编号：BD-560164      品牌：BD-Pharmingen       原厂货号：560164
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

cAMP-dependent Protein Kinase (PKA) is composed of two distinct subunits: catalytic (C) and regulatory (R). Four regulatory subunits have
been identified: RIα, RIβ, RIIα, and RIIβ. These subunits define type I and II PKAs. Following binding of cAMP, the regulatory subunits
dissociate from the catalytic subunits, rendering the enzyme active. Type I and II holoenzymes have three potential C subunits (Cα, Cβ, or
Cγ). Type II PKA can be distinguished by autophosphorylation of the R subunits, while type I PKA binds Mg/ATP with high affinity. The
cAMP-dependent autophosphorylation of the human RIIα subunit occurs at Serine 99 (S99) [Entrez Protein Accession #CAA33094]. Most
cells express both type I and type II PKAs. Although the Rα isoforms are ubiquitously expressed, the Rβ isoforms are predominantly found
in nervous and adipose tissues. In addition to their enzyme regulatory activity, the RIIα and RIIβ subunits determine the subcellular location
of the holoenzymes via their interactions with specific intracellular anchoring proteins.
The I65-856.286 antibody recognizes human PKA RIIα phosphorylated at S99.
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原厂资料：

注意事项：

1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-μl experimental
sample (a test).
2.Since applications vary, each investigator should titrate the reagent to obtain optimal results..
3.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before
discarding to avoid accumulation of potentially explosive deposits in plumbing.
4.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.