CD22 (pY822) Alexa 488 12a/CD22 50Tst

• 产品编号：BD-560075      品牌：BD-Pharmingen       原厂货号：560075
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

CD22 is a member of the immunoglobulin (Ig) superfamily that contains several extracellular Ig domains and cytoplasmic Immunoreceptor
Tyrosine-based Inhibitory Motifs (ITIMs). It is expressed in the cytoplasm of pro-B and pre-B cells and on the surface of IgD-positive mature
B lymphocytes. Differential splicing of the human CD22 gene results in the expression of two isoforms; the full-length CD22b has 7 Ig
domains, while CD22a has only 5. CD22b is the predominant isoform in human cells, at both the mRNA and protein levels. Ligands for the
extracellular domains of CD22 are sialic acid-linked glycoproteins on the B cell surface. The intracellular ITIM motifs are substrates of src
family tyrosine kinases. Signal transduction is initiated by the phosphorylation of tyrosines in the ITIMs of CD22 upon ligation of the B cell
receptor (BCR). Tyrosine phosphorylation in ITIM3 of human CD22 or ITIM2 of mouse CD22 is required for recruitment of the protein
tyrosine phosphatase PTP1C (SHP-1), which may terminate signal transduction by CD22 and by co-localized receptors, such as the BCR.
Studies of mice deficient for CD22 suggest that this adhesion molecule generates both negative and positive regulatory signals that affect B
cell development and responsiveness.
The 12a/CD22 monoclonal antibody recognizes the phosphorylated tyrosine 822 (pY822) in ITIM3 of human CD22b. The equivalent site in
human CD22a is pY645. The orthologous phosphorylation site in the predominant isoform of mouse CD22 is pY837, which is in ITIM2.
We recommend the PE conjugate of mAb 12a/CD22, Cat. No. 560076, for staining human peripheral blood mononuclear cells (PBMC).

原厂资料：

注意事项：

1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-μl experimental
sample (a test).
2.Since applications vary, each investigator should titrate the reagent to obtain optimal results..
3.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before
discarding to avoid accumulation of potentially explosive deposits in plumbing.
4.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.