Ms CD137 PE 1AH2 100ug

• 产品编号：BD-558976      品牌：BD-Pharmingen       原厂货号：558976
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

The 1AH2 clone (subclone of 53A2) has been reported to react with CD137, a member of the TNFR/NGFR superfamily. The expression of
CD137 has been reported to be detectable from day 3 and peaks around day 6 after activation with ConA, PMA plus ionomycin, or
immobilized anti-CD3e (Cat. No. 553058). Monomers, dimers, or tetramers of the 4-1BB antigen are expressed, upon activation, on the
surface of splenic T lymphocytes, thymocytes, intestinal intraepithelial T lymphocytes (IEL), and some T cell lines and clones. While
stimulating T cells by IL-2, IL-4, or anti-CD28 alone does not result in the expression of CD137; addition of IL-2, IL-4, anti-CD28, or
syngeneic accessory cells to splenic T cells stimulated via TCR/CD3 can result in a high level of CD137 expression. CD137 has also been
reported to be observed on IL-2 activated NK cells, but not on freshly isolated NK cells. It has been demonstrated that 4-1BB physically
associates with p56 [lck]through a Cys-Arg-Cys-Pro binding site in its cytoplasmic domain; the same motif in the cytoplasmic tail of the CD4
and CD8a molecules is responsible for association with p56 [lck]. A signaling function for the CD137 molecule in mouse T cells is indicated
by reports in which cross-linking of CD137 with 1AH2 mAb resulted in enhanced proliferation of CD3e-activated splenic T cells and IEL and
in enhanced cytolytic activity of IEL in response to immobilized anti-CD3e. In addition to extracellular matrix proteins which bind to CD137,
a 97-kDa dimer of the TNF/NGF superfamily has been reported to be a ligand for 4-1BB (4-1BBL). This molecule has been detected on Con
A-activated T cells, LPS-activated macrophages, and anti-μ-activated splenic B cells. Interaction between T and B cells through
4-1BB/4-1BBL is reported to play a role in antigen presentation, further supporting a costimulatory role for CD137 in the immune response of
T lymphocytes.

原厂资料：

注意事项：

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3.The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
4.The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
5.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.