Red FP Vector Peroxisome 20ug

产品编号：BD-558724 品牌：BD-Pharmingen 原厂货号：558724
产品分类：克隆 > 克隆载体和克隆试剂盒 > 载体 > 报告基因载体
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Store undiluted at -20°C.

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描述：

BD Pharmingen™ Red FP Vector - Peroxisome is a mammalian expression vector that encodes a fusion of the red fluorescent protein (FP) from Discosoma sp. with the peroxisomal targeting signal 1 (PTS1), which encodes a tripeptide SKL that targets the red FP to the matrix of peroxisomes. The PTS1 sequence is fused to the 3’-end of red FP. In order to increase the translation efficiency in mammalian cells, a Kozak consensus translation initiation site has been introduced to the 5’-end of the red FP open reading frame, and its sequence has been optimized with human codons. The red FP fusion is expressed under the control of the immediate early promoter of cytomegalovirus (P CMV ie), and its sequence is followed by downstream simian virus 40 (SV40) polyadenylation signals. The vector contains a neomycin resistance gene controlled by the SV40 promoter (P SV40 e) that allows selection of stably transfected eukaryotic cells using G418. An additional bacterial (Kan R) promoter drives the expression of the same gene encoding for kanamycin resistance in E. coli.

注意事项：

Store undiluted at -20°C.