BD Pharmingen™ Green FP Vector - Actin is a mammalian expression vector that encodes a fusion of the green fluorescent protein (FP) from
Aequorea coerulescens with the the human cytoplasmic β-actin. The human β-actin sequence is fused to the 3’-end of green FP. In order to
increase the translation efficiency in mammalian cells, a Kozak consensus translation initiation site has been introduced to the 5’-end of the
green FP open reading frame, and its sequence has been optimized with human codons. The green FP fusion is expressed under the control of
the immediate early promoter of cytomegalovirus (P CMV ie), and its sequence is followed by downstream simian virus 40 (SV40)
polyadenylation signals. The vector contains a neomycin resistance gene controlled by the SV40 promoter (P SV40 e) that allows selection of
stably transfected eukaryotic cells using G418. An additional bacterial (Kan R) promoter drives the expression of the same gene encoding for
kanamycin resistance in E. coli.
京公网安备11010802025653 版权所有：北京逸优科技有限公司
