Green FP Vector Nucleus 20ug

产品编号：BD-558720 品牌：BD-Pharmingen 原厂货号：558720
产品分类：克隆 > 克隆载体和克隆试剂盒 > 载体 > 报告基因载体
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Store undiluted at -20°C.

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描述：

BD Pharmingen™ Green FP Vector - Nucleus is a mammalian expression vector that encodes a fusion of the green fluorescent protein (FP) from Aequorea coerulescens with three copies of the nuclear localization signal (NLS) of the simian virus 40 (SV40) large T antigen. The NLS sequences are fused to the 3’-end of green FP. In order to increase the translation efficiency in mammalian cells, a Kozak consensus translation initiation site has been introduced to the 5’-end of the green FP open reading frame, and its sequence has been optimized with human codons. The green FP fusion is expressed under the control of the immediate early promoter of cytomegalovirus (P CMV ie), and its sequence is followed by downstream SV40 polyadenylation signals. The vector contains a neomycin resistance gene controlled by the SV40 promoter (P SV40 e) that allows selection of stably transfected eukaryotic cells using G418. An additional bacterial (Kan R) promoter drives the expression of the same gene encoding for kanamycin resistance in E. coli.

注意事项：

Store undiluted at -20°C.