Green FP Vector Golgi 20ug

产品编号：BD-558719 品牌：BD-Pharmingen 原厂货号：558719
产品分类：克隆 > 克隆载体和克隆试剂盒 > 载体 > 报告基因载体
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Store undiluted at -20°C.

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描述：

BD Pharmingen™ Green FP Vector - Golgi is a mammalian expression vector that encodes a fusion of the green fluorescent protein (FP) from Aequorea coerulescens with the first 81 N-terminal amino acids of the human beta1,4-galactosyltransferase. The membrane-anchoring signal peptide sequence from the human beta 1,4-galactosyltransferase is fused to the 5’-end of green FP. In order to increase the translation efficiency in mammalian cells, a Kozak consensus translation initiation site has been introduced to the 5’-end of the green FP open reading frame, and its sequence has been optimized with human codons. The green FP fusion is expressed under the control of the immediate early promoter of cytomegalovirus (P CMV ie), and its sequence is followed by downstream simian virus 40 (SV40) polyadenylation signals. The vector contains a neomycin resistance gene controlled by the SV40 promoter (P SV40 e) that allows selection of stably transfected eukaryotic cells using G418. An additional bacterial (Kan R) promoter drives the expression of the same gene encoding for kanamycin resistance in E. coli.

注意事项：

Store undiluted at -20°C.