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Hu CD11c IMag-DM S-HCL-3 5mL

 
包装: 5mL
运保温度: Store undiluted at 4°C
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描述:

BD IMag™ anti-human CD11c Particles - DM are magnetic nanoparticles that have monoclonal antibody conjugated to their surfaces. These
particles are optimized for the positive selection or depletion of CD11c-bearing cells using the BD IMagnet™. The CD11c antigen is present
on monocytes and in low density on granulocytes and natural killer (NK) cells and dendritic cells (DC) in peripheral blood. It is also present
on hairy-cell leukemias and on some acute myeloid leukemias (AML). CD11c reacts with an epitope on the α (150-kDa) chain of the
CD11c/CD18 antigen complex. In addition, CD11c stains sinus histiocytes of lymph node, tonsil, and spleen; macrophages in the cortex and
medulla of thymus; Kupffer cells in liver; elongated intertubular cells in kidney; and alveolar macrophages in lung tissue. In malignant tissue,
the antibody stains true histiocytic lymphomas; it does not stain carcinomas or T or B lymphomas.
PBMCs are labeled with BD IMag™ anti-human CD11c Magnetic Particles - DM according to the following Magnetic Labeling Protocol.
This labeled cell suspension is then placed within the magnetic field of the BD IMagnet™ (Cat. no. 552311). Labeled cells migrate toward
the magnet (positive fraction), leaving the unlabeled cells in suspension so they can be drawn off (negative fraction). The tube is then
removed from the magnetic field for resuspension of the positive fraction. The separation is repeated twice to increase the purity of the
positive fraction. The magnetic separation steps are diagrammed in the Separation Flow Chart. After the positive fraction is washed, the small
size of the magnetic particles allows the positive fraction to be further evaluated in downstream applications such as flow cytometry. This kit
can be used in conjunction with the Human Dendritic Cell Enrichment Set - DM (Cat. No. 558420) to select for an enriched myeloid DC
population (CD123 negative; CD11c positive) or plasmacytoid DC population (CD123 positive; CD11c negative).


注意事项:

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. .
3.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.


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