TBK1 PE 637Ig11.2 50Tst

• 产品编号：BD-558696      品牌：BD-Pharmingen       原厂货号：558696
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

NF-kB is a ubiquitously expressed transcription factor that regulates many cytokine and Ig genes. It is involved in immune, inflammatory,
viral, and acute phase responses. In most cells, NF-kB is sequestered in an inactive cytoplasmic form via interactions with the inhibitory
proteins IkBα, IkBβ, and IkBε. Stimulation induces the release, activation, and nuclear translocation of NF-kB. Release of NF-kB results
from the phosphorylation and proteolytic degradation of the IkB proteins. Two cytokine-inducible IκB kinases (IKKa and IKKb)
phosphorylate and target the IkB proteins for degradation via the ubiquitin pathway. IKKg/NEMO, a third member of the IKK complex,
functions as a regulatory subunit and interacts directly with IKKb. TBK1 (TANK-binding kinase 1, also known as T2K or NAK), a protein
of 729 amino acids, is another member of the IKK family of kinases regulating NF-kB downstream of the tumor necrosis factor and Toll-like
receptor pathways. TBK1 forms a complex with the adaptor proteins TANK (TRAF-associated NF-kB activator) and TRAF2
(TNF-receptor-associated factor 2), and this oligomer is required for activation and phosphorylation of TBK1 at serine 172.
The 637Ig11.2 monoclonal antibody recognizes human TBK1, regardless of phosphorylation status. Our in-house testing is performed on a
cell line that has been co-transfected with TBK1 and IRF-7 because we have been unable to detect endogenous TBK1 by western blotting of
lysates from activated cell lines (HeLa activated with PMA and A431 activated with PDGF). In the transfectants, the over-expression of
TBK1 is necessary for phosphorylation of IRF-7, but the presence of IRF-7 does not affect TBK1 expression or phosphorylation. We
confirmed that mAb 637Ig11.2 does not cross-react with IRF-7 by western blot analysis using the purified antibody.

原厂资料：

注意事项：

1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 X 10e6 cells in a 100-μl experimental sample (a test).
2.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
5.The antibody was conjugated with APC under optimum conditions, and unconjugated antibody and free APC were removed.