p120 Catenin (pT310) Alexa 488 50Tst

• 产品编号：BD-558667      品牌：BD-Pharmingen       原厂货号：558667
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

The membrane-associated protein pp120 Src substrate (p120 catenin, p120cas) was identified as a tyrosine kinase substrate that is phosphorylated in Src-transformed cells. p120 catenin is localized to the E-Cadherin/catenin cell adhesion complex. Like b- and g-catenin, p120 catenin directly associates with the cytoplasmic C terminus of E-Cadherin via its Arm domain. It exists as four isoforms that range in size from 90 to 115 kDa. Expression of these isoforms is heterogeneous in human carcinomas, suggesting that altered expression contributes to malignancy. Phosphorylation of multiple serine (S252, S268, S288, and S879) and threonine (T310 and T916) residues in p120 catenin may regulate its activity. The S879 residue is phosphorylated after PKC activation, while the S268 site is dephosphorylated after PKC activation. The latter residue is phosphorylated in vitro by p160 Rock. S252 and T310 residues are phosphorylated in vitro by GSK3b. Thus, p120 catenin function may be regulated in a complex manner through both serine and threonine phosphorylation. The 22/p120 (pT310) monoclonal antibody recognizes the phosphorylated T310 in the regulatory domain of p120 catenin.

原厂资料：

注意事项：

1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-μl experimental sample (a test). 2.Since applications vary, each investigator should titrate the reagent to obtain optimal results.. 3.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. 4.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.