IRF-7(pS477/pS479)Alexa488 K47-671 50Tst

产品编号：BD-558629 品牌：BD-Pharmingen 原厂货号：558629
产品分类：抗体 > 一抗 > 染料标记抗体
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包装：

50Tst

运保温度:

Store undiluted at 4°C

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描述：

Interferon regulatory factor 7 (IRF-7) is a transcription factor that regulates anti-viral defenses by controlling the induction of type-I interferon (IFN) responses. IRF-7 expression is induced in lymphoid cells by virus infection, as well as by IFN, lipopolysaccharide, and TNF-a. IRF-7 responses are initiated by Toll-like receptors (TLR) or the cytoplasmic protein retinoic acid inducible gene I (RIG-I). Upon TLR activation, it forms cytoplasmic complexes with MyD88, an adaptor in the TLR signaling pathways. The TLR-dependent and RIG-I-dependent pathways activate kinases, such as IKK-e and TBK1, that phosphorylate IRF-7 and induce movement of IRF-7-containing complexes to the nucleus, where it preferentially activates IFN-a promoters. The K47-671 monoclonal antibody recognizes human IRF-7 phosphorylated at serines 477 and 479 (pS477/pS479). Our in-house testing is performed on a cell line that has been co-transfected with TBK1 and IRF-7. Phosphorylation of IRF-7 in the transfectants requires virus infection or over-expression of a signaling molecule of the RIG-I pathway, such as TBK1. Phosphorylation of endogenous IRF-7 in untransfected cells has not yet been detected. We confirmed that mAb K47-671 does not cross-react with TBK1 by Western blot analysis using the purified antibody.

注意事项：

1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-μl experimental sample (a test). 2.Since applications vary, each investigator should titrate the reagent to obtain optimal results.. 3.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. 4.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.