Functional Bead Conjugation Buffer Set

• 产品编号：BD-558556      品牌：BD-Pharmingen       原厂货号：558556
• 产品分类：ELISA及其它免疫分析 > 其它免疫检测 > CBA基于微球的免疫分析-流式细胞术
• 应用分类：

描述：

Flow cytometry is an analysis tool that allows for the discrimination of different particles on the basis of size and fluorescence. The BD™ CBA Functional Bead system consists of different color-coded 7.5 μm polystyrene beads that can be classified using a flow cytometer (Refer to Appendix, table 1 for examples of compatible instrument platforms). The Functional Bead Conjugation Buffer Set contains the supporting reagents necessary to covalently link water-soluble proteins to the surface of functional beads. The beads, once linked to a protein, can be used as a capture matrix for analytes of interest. The Functional Bead Conjugation Buffer Set contains enough material for 15 coupling reactions. Each coupling reaction yields approximately 3 – 6 ×106 beads which is enough material to run 500 or 1000 tests at a concentration of 6,000 beads per well. For a 500 test conjugation reaction 90 μg of protein is needed, and for a 1000 test conjugation reaction 180 μg of protein is needed. After covalently linking protein to the beads, it is recommended that a test be performed to confirm the success of the reaction. For that purpose, BD Biosciences offers three different phycoerythrin (PE) Functional Bead Ig Detectors that can be used to confirm the presence of antibody covalently linked to the beads. (See page 8 for ordering information). If another type of protein was coupled to the functional beads, please refer to the Conjugation Confirmation section (pg. 11) for options.

原厂资料：

注意事项：

• For Research Use Only. • Not recommended for use on stream-in-air instruments where signal intensities may be reduced. Stream-in-air instruments include the BD FACStar™ Plus and the BD FACSVantage™ flow cytometers. • Protein must be purified and free of BSA, glycine, Tris, or any other proteins or protein stabilizing additives. The protein must be suspended in PBS, pH 7.2 ± 0.2 before attempting the coupling reaction. • Not suitable for conjugation of ascites or crude antiserum. • Although suitable for conjugation of any protein with a free amino group, the protocol has only been validated for IgG subclass antibodies. • Functional Beads are stable at 4°C for 1 year after conjugation. • Do not use expired reagents.