Btk PE 53/Btk 50Tst

• 产品编号：BD-558527      品牌：BD-Pharmingen       原厂货号：558527
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

Bruton's tyrosine kinase (Btk) is a nonreceptor tyrosine kinase whose function is critical for proper B cell development and signaling. It is a
member of the Tec family of kinases which includes Tec and Itk. This family is similar to the src family of tyrosine kinases. However, Tec
family members lack the N-terminal myristylation site and the regulatory C-terminal tyrosine that are found in src proteins. In addition to an
N-terminal pleckstrin homology (PH) domain, the Tec proteins contain Src homology domains 2 and 3 (SH2 and SH3) and a stretch of 60-80
amino acids between the PH and SH3 domains termed the Tec homology domain. The activity of Btk is regulated by Src-mediated
phosphorylation of the kinase domain at tyrosine 551. This event induces Btk kinase activity and subsequent autophosphorylation at tyrosine
223 in the SH3 domain. Phosphorylated Btk then associates with the cell membrane via the interaction of the PH domain with
phosphatidylinositol 3, 4, 5-triphosphate. The PH domain is essential for proper activation and function of Btk. A mutation in the PH domain
results in Xid, murine X-linked immunodeficiency, and human X-linked agammaglobulinemia.

原厂资料：

注意事项：

1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 X 10e6 cells in a 100-μl experimental sample (a test).
2.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
5.The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.